Immunoassay is a sensitive analytical technique used to detect and quantify specific molecules in a sample by utilizing the highly selective binding between an antigen and an antibody. This technique is widely employed in medical diagnostics, pharmaceuticals, environmental monitoring, and food safety. Immunoassays can detect various analytes, including proteins, hormones, drugs, and pathogens, with high specificity and sensitivity. Different immunoassay formats exist, such as enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (RIA), and lateral flow assays (LFAs), each offering unique advantages in terms of sensitivity, speed, and simplicity. ELISA, for example, utilizes enzyme-labeled antibodies to generate a detectable signal, while LFAs provide rapid, qualitative results suitable for point-of-care testing. Immunoassays play a crucial role in disease diagnosis, monitoring treatment efficacy, and screening for infectious agents. Continuous advancements in immunoassay technology, including multiplexing and miniaturization, enable high-throughput analysis and the detection of multiple analytes simultaneously. Collaboration between researchers, clinicians, and industry professionals drives innovation in immunoassay development, expanding its applications and improving its performance for various analytical needs.