Targeting out of range biomolecules: Chemical labeling strategies for qualitative and quantitative MS-based detection

Title : Targeting out of range biomolecules: Chemical labeling strategies for qualitative and quantitative MS-based detection

Abstract:

In health sciences and particularly in –omics approaches, Mass spectrometry is of strategic importance for the detection and identification of specific molecules involved in biological processes, leading to a better understanding of physiological and physiopathological processes and consequently for diagnosis purposes. However, a large number of these relevant biomarkers remain undetectable due to several parameters, a low molecular weight, a weak abundance, their ionization potential or their localization in a complex environment. To overcome these limitations, labeling strategies have been developed. MS labeling require the development of diverse tags as versatile as possible, involving innovative chemistry, and targeting different functional groups such as amine, hydroxy groups or carboxylic acids. Specific probes, such as ABP have been designed to selectively label biomolecules of interest and to match the current need for in vivo labeling methods following the development of imaging techniques.

Audience Take Away Notes

• Chemical development in currently highly-relevant topic of chemical labeling, which allows overcoming the limitations of the MALDI MS, generally considered as a label-free technique.
• Different labeling strategies for MALDI-MS are presented MALDI-MS is a key technique for biomolecule gentle identification and proteomics.This talk supplies a description of derivatization methods while putting into perspective the importance of MALDI labels in the bioresearch context by targeting out of range biomolecules in complex media.
• Yes through chemical derivatization, compatible with biological media.
• Yes by designing specific probes for detection of post translational modification hard to detect.

Biography:

Sonia Cantel obtained her PhD in 2004 in Chemistry of Biomolecules from the University of Montpellier, in the field of pseudo-peptide and solid phase organic synthesis. For the following two years as Post-doctoral Fellow in the Laboratory for Translational Research (Harvard Medical School, Boston, USA), she actively participated to biomedicinal projects and developed extensive skills in analytical techniques applied to peptide and protein engineering. She joined in 2007 the IBMM, taking advantage of her multidisciplinary experience to develop new projects at the interface of Chemistry, Biology and Analytical Sciences.

She focuses her research on the development of chemical probes for specific and sensitive detection of peptides and proteins, and pharmacological studies (GPCR/ligand interaction) by MALDI Mass Spectrometry. Actively involved in peptide environment, she is currently at the directing board of the GFPP (French Group of peptides and proteins). She has published more than 50 research articles in SCI(E) journals h-index:16.